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hinge 1 region  (Developmental Studies Hybridoma Bank)
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rat anti mouse macrophage  (ATCC)
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ATCC rat anti mouse macrophage
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anti viral therapies  (Gilead Sciences)
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mouse monoclonal antibodies  (Jackson Immuno)
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Jackson Immuno mouse monoclonal antibodies
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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peroxidase conjugated donkey anti goat  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology peroxidase conjugated donkey anti goat
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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goat anti human igg  (Bio-Rad)
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Bio-Rad goat anti human igg
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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human phosphorjnk1 þ jnk2  (Cell Signaling Technology Inc)
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FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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anti fak  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti fak
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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p53  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology p53
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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mouse anti-human-fxr monoclonal antibody  (Perseus Proteomics)
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Perseus Proteomics mouse anti-human-fxr monoclonal antibody
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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caspase 8  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc caspase 8
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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goat anti rabbit alkaline phosphatase secondary antibody  (Jackson Immuno)
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Jackson Immuno goat anti rabbit alkaline phosphatase secondary antibody
FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 <t>monoclonal</t> antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.
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FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 monoclonal antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.

Journal: Urology

Article Title: Preliminary immunohistochemical characterization of a monoclonal antibody (pro:4-216) prepared from human prostate cancer nuclear matrix proteins

doi: 10.1016/s0090-4295(97)00337-3

Figure Lengend Snippet: FIGURE 2. NMP composition of normal human prostate (A) and human prostate cancer (C) taken from a radical prostatectomy specimen from a patient with clinical/y localized prostate cancer. High resolution two-dimensional gel electrophoresis of NMP preparations were isolated and analyzed as described in Methods. Panels B and D represent Western immunoblots using PRO:4-2 16 monoclonal antibody; immunoblotting technique as described in Methods. Arrowhead represents the localization of NMP(s) with immunoreactivity to the PRO:4-2 16 mouse monoclonal anti- body generated against human prostate cancer nuclear matrix preparations. kD = molecular weight in thousands; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis; pl = isoelectric point.

Article Snippet: The mouse monoclonal antibodies were UROLOGY 50 (51, 1997 801 then detected with a CY3 conjugated goat anti-mouse antibody (Jackson ImmunoResearch, West Grove, Pa) and DNA was visualized with DAPI (4,6-diamidino-2-phenylindole) at 2 /.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Isolation, Western Blot, Generated, Molecular Weight, SDS Page, Polyacrylamide Gel Electrophoresis

FIGURE 3. lmmunohistochemical staining of human prostate tissue with the mouse monoclonal antibody PRO:4- 2 16. Anti-mouse IgM secondary antibody (Vector’s ABC Elite Kit). 3-3’-Diaminobenzidine tetrahydrochloride (DAB) was used as a chromogen following ethyl green counterstain. (A) Histologically normal prostate tissue (nuclei dem- onstrate an IHC intensity score of 0). (B) Histologically normal prostate tissue with the emphasis on stromal nuclei (weakly positively staining stromal nuclei represent an IHC intensity score of 1). (Cl Adenocarcinoma of the prostate from a tumor with Gleason grade 3 + 3 = 6 (IHC intensity score of 3). The cancerous glands surround a noncancerous gland. (0) A prostatic gland demonstrating PIN (positively staining nuclei represent an IHC intensity score of 1 to 2). The arrow in panel B identifies an area of prostatic stroma. The small arrows in panel C identify prostatic ade- nocarcinoma glands and the large arrow in panel C identifies a histologically normal gland found among cancerous glands. The arrow in panel D represents histologic PIN.

Journal: Urology

Article Title: Preliminary immunohistochemical characterization of a monoclonal antibody (pro:4-216) prepared from human prostate cancer nuclear matrix proteins

doi: 10.1016/s0090-4295(97)00337-3

Figure Lengend Snippet: FIGURE 3. lmmunohistochemical staining of human prostate tissue with the mouse monoclonal antibody PRO:4- 2 16. Anti-mouse IgM secondary antibody (Vector’s ABC Elite Kit). 3-3’-Diaminobenzidine tetrahydrochloride (DAB) was used as a chromogen following ethyl green counterstain. (A) Histologically normal prostate tissue (nuclei dem- onstrate an IHC intensity score of 0). (B) Histologically normal prostate tissue with the emphasis on stromal nuclei (weakly positively staining stromal nuclei represent an IHC intensity score of 1). (Cl Adenocarcinoma of the prostate from a tumor with Gleason grade 3 + 3 = 6 (IHC intensity score of 3). The cancerous glands surround a noncancerous gland. (0) A prostatic gland demonstrating PIN (positively staining nuclei represent an IHC intensity score of 1 to 2). The arrow in panel B identifies an area of prostatic stroma. The small arrows in panel C identify prostatic ade- nocarcinoma glands and the large arrow in panel C identifies a histologically normal gland found among cancerous glands. The arrow in panel D represents histologic PIN.

Article Snippet: The mouse monoclonal antibodies were UROLOGY 50 (51, 1997 801 then detected with a CY3 conjugated goat anti-mouse antibody (Jackson ImmunoResearch, West Grove, Pa) and DNA was visualized with DAPI (4,6-diamidino-2-phenylindole) at 2 /.

Techniques: Staining